PhytophthoraV1, Main, Exploration, bibRecord, 000559

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.

Identifieur interne : 000559 ( Main/Exploration ); précédent : 000558; suivant : 000560

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.

Auteurs : Weidan Chang [République populaire de Chine] ; Weipeng Liu [République populaire de Chine] ; Ying Liu [République populaire de Chine] ; Fangfang Zhan [République populaire de Chine] ; Huifang Chen [République populaire de Chine] ; Hongtao Lei [République populaire de Chine] ; Yingju Liu [République populaire de Chine]

Source :

Mikrochimica acta [ 1436-5073 ] ; 2019.

RBID : pubmed:30877395

Descripteurs français

KwdFr :
- ADN (analyse), ADN (génétique), Colorimétrie (méthodes), Hybridation d'acides nucléiques (MeSH), Limite de détection (MeSH), Nanoparticules métalliques (composition chimique), Oligodésoxyribonucléotides (composition chimique), Oligodésoxyribonucléotides (génétique), Or (composition chimique), Phytophthora infestans (génétique), Protéine-9 associée à CRISPR (composition chimique), Systèmes CRISPR-Cas (MeSH), Séquence nucléotidique (MeSH), Techniques d'amplification d'acides nucléiques (méthodes), Techniques de biocapteur (méthodes).
MESH :
- analyse : ADN.
- composition chimique : Nanoparticules métalliques, Oligodésoxyribonucléotides, Or, Protéine-9 associée à CRISPR.
- génétique : ADN, Oligodésoxyribonucléotides, Phytophthora infestans.
- méthodes : Colorimétrie, Techniques d'amplification d'acides nucléiques, Techniques de biocapteur.
- Hybridation d'acides nucléiques, Limite de détection, Systèmes CRISPR-Cas, Séquence nucléotidique.

English descriptors

KwdEn :
- Base Sequence (MeSH), Biosensing Techniques (methods), CRISPR-Associated Protein 9 (chemistry), CRISPR-Cas Systems (MeSH), Colorimetry (methods), DNA (analysis), DNA (genetics), Gold (chemistry), Limit of Detection (MeSH), Metal Nanoparticles (chemistry), Nucleic Acid Amplification Techniques (methods), Nucleic Acid Hybridization (MeSH), Oligodeoxyribonucleotides (chemistry), Oligodeoxyribonucleotides (genetics), Phytophthora infestans (genetics).
MESH :
- chemical , analysis : DNA.
- chemical , chemistry : CRISPR-Associated Protein 9, Gold, Oligodeoxyribonucleotides.
- chemical , genetics : DNA, Oligodeoxyribonucleotides.
- chemistry : Metal Nanoparticles.
- genetics : Phytophthora infestans.
- methods : Biosensing Techniques, Colorimetry, Nucleic Acid Amplification Techniques.
- Base Sequence, CRISPR-Cas Systems, Limit of Detection, Nucleic Acid Hybridization.

Abstract

A colorimetric method is presented for the detection of specific nucleotide sequences in plant pathogens. It is based on the use of CRISPR/Cas9-triggered isothermal amplification and gold nanoparticles (AuNPs) as optical probes. The target DNA was recognized and broken up by a given Cas9/sgRNA complex. After isothermal amplification, the product was hybridized with oligonucleotide-functionalized AuNPs. This resulted in the aggregation of AuNPs and a color change from wine red to purple. The visual detection limit is 2 pM of DNA, while a linear relationship exists between the ratio of absorbance at 650 and 525 nm and the DNA concentration in the range from 0.2 pM to 20 nM. In contrast to the previous CRISPR-based amplification platforms, the method has significantly higher specificity with the single-base mismatch and can be visually read out. It was successfully applied to identify the Phytophthora infestans genomic DNA. Graphical abstract Schematic presentation of a colorimetric method for detection of Phytophthora infestans genomic DNA based on CRISPR/Cas9-triggered isothermal amplification. The Cas9 endonuclease cleaves DNA at the design site and the color changes from red to purple with increasing target DNA concentration.

DOI: 10.1007/s00604-019-3348-2
PubMed: 30877395

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000523
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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term>
<term>Biosensing Techniques (methods)</term>
<term>CRISPR-Associated Protein 9 (chemistry)</term>
<term>CRISPR-Cas Systems (MeSH)</term>
<term>Colorimetry (methods)</term>
<term>DNA (analysis)</term>
<term>DNA (genetics)</term>
<term>Gold (chemistry)</term>
<term>Limit of Detection (MeSH)</term>
<term>Metal Nanoparticles (chemistry)</term>
<term>Nucleic Acid Amplification Techniques (methods)</term>
<term>Nucleic Acid Hybridization (MeSH)</term>
<term>Oligodeoxyribonucleotides (chemistry)</term>
<term>Oligodeoxyribonucleotides (genetics)</term>
<term>Phytophthora infestans (genetics)</term>
</keywords>
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<term>ADN (génétique)</term>
<term>Colorimétrie (méthodes)</term>
<term>Hybridation d'acides nucléiques (MeSH)</term>
<term>Limite de détection (MeSH)</term>
<term>Nanoparticules métalliques (composition chimique)</term>
<term>Oligodésoxyribonucléotides (composition chimique)</term>
<term>Oligodésoxyribonucléotides (génétique)</term>
<term>Or (composition chimique)</term>
<term>Phytophthora infestans (génétique)</term>
<term>Protéine-9 associée à CRISPR (composition chimique)</term>
<term>Systèmes CRISPR-Cas (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Techniques d'amplification d'acides nucléiques (méthodes)</term>
<term>Techniques de biocapteur (méthodes)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>CRISPR-Associated Protein 9</term>
<term>Gold</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Nanoparticules métalliques</term>
<term>Oligodésoxyribonucléotides</term>
<term>Or</term>
<term>Protéine-9 associée à CRISPR</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora infestans</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term>
<term>Oligodésoxyribonucléotides</term>
<term>Phytophthora infestans</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
<term>Colorimetry</term>
<term>Nucleic Acid Amplification Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Colorimétrie</term>
<term>Techniques d'amplification d'acides nucléiques</term>
<term>Techniques de biocapteur</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>CRISPR-Cas Systems</term>
<term>Limit of Detection</term>
<term>Nucleic Acid Hybridization</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Hybridation d'acides nucléiques</term>
<term>Limite de détection</term>
<term>Systèmes CRISPR-Cas</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">A colorimetric method is presented for the detection of specific nucleotide sequences in plant pathogens. It is based on the use of CRISPR/Cas9-triggered isothermal amplification and gold nanoparticles (AuNPs) as optical probes. The target DNA was recognized and broken up by a given Cas9/sgRNA complex. After isothermal amplification, the product was hybridized with oligonucleotide-functionalized AuNPs. This resulted in the aggregation of AuNPs and a color change from wine red to purple. The visual detection limit is 2 pM of DNA, while a linear relationship exists between the ratio of absorbance at 650 and 525 nm and the DNA concentration in the range from 0.2 pM to 20 nM. In contrast to the previous CRISPR-based amplification platforms, the method has significantly higher specificity with the single-base mismatch and can be visually read out. It was successfully applied to identify the Phytophthora infestans genomic DNA. Graphical abstract Schematic presentation of a colorimetric method for detection of Phytophthora infestans genomic DNA based on CRISPR/Cas9-triggered isothermal amplification. The Cas9 endonuclease cleaves DNA at the design site and the color changes from red to purple with increasing target DNA concentration.</div>
</front>
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<Abstract><AbstractText>A colorimetric method is presented for the detection of specific nucleotide sequences in plant pathogens. It is based on the use of CRISPR/Cas9-triggered isothermal amplification and gold nanoparticles (AuNPs) as optical probes. The target DNA was recognized and broken up by a given Cas9/sgRNA complex. After isothermal amplification, the product was hybridized with oligonucleotide-functionalized AuNPs. This resulted in the aggregation of AuNPs and a color change from wine red to purple. The visual detection limit is 2 pM of DNA, while a linear relationship exists between the ratio of absorbance at 650 and 525 nm and the DNA concentration in the range from 0.2 pM to 20 nM. In contrast to the previous CRISPR-based amplification platforms, the method has significantly higher specificity with the single-base mismatch and can be visually read out. It was successfully applied to identify the Phytophthora infestans genomic DNA. Graphical abstract Schematic presentation of a colorimetric method for detection of Phytophthora infestans genomic DNA based on CRISPR/Cas9-triggered isothermal amplification. The Cas9 endonuclease cleaves DNA at the design site and the color changes from red to purple with increasing target DNA concentration.</AbstractText>
</Abstract>
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<ForeName>Weidan</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>College of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.</Affiliation>
</AffiliationInfo>
</Author>
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<ForeName>Weipeng</ForeName>
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</AffiliationInfo>
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</AffiliationInfo>
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<ForeName>Fangfang</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Chen</LastName>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Lei</LastName>
<ForeName>Hongtao</ForeName>
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<AffiliationInfo><Affiliation>The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Yingju</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>College of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China. liuyingju@hotmail.com.</Affiliation>
</AffiliationInfo>
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<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>21705051</GrantID>
<Agency>National Natural Science Foundation of China</Agency>
<Country>International</Country>
</Grant>
<Grant><GrantID>21874048</GrantID>
<Agency>National Natural Science Foundation of China</Agency>
<Country>International</Country>
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Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.

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